Sabtu, 25 Juni 2016

PRAKTIKUM BIOLOGI SEL MOLEKULER PERCOBAAN I ANALISIS GEN DAN HOMOLOGI PROTEIN

LAPORAN RESMI
PRAKTIKUM BIOLOGI SEL MOLEKULER
PERCOBAAN I
ANALISIS GEN DAN HOMOLOGI PROTEIN

Dosen Pengampu : Ibrahim Arifin,Msc,.Apt


Disusun Oleh :





UNIVERSITAS WAHID HASYIM
SEMARANG
2014



























HASIL PENGAMATAN
I.LATIHAN
Kode Gen       : NM_000125
Nama Gen       : Homosapiens Estrogen Receptor-1
CDS                : 235...2022
Frame
Panjang
Start Kodon
Stop Kodon
+1
1....1787
27
ATG
+2
506...751
4
TGA
+2
1610....1787
2
TGA
+3
3....254
-
TGA
+3
1248....1364
1
TAA
-1
1572....1294
2
TGA
-2
735....977
3
TAG
-2
1583...1583
9
TGA
-3
299....445
1
TGA

Kode Gen       :  NM_000125
Nama Gen       : Homosapiens Estrogen Receptor-1
Kode Protein   : NP_000162.2
CDS                : 1....595
DESKRIPSI
MAX SCORE
estrogen receptor isoform 1 [Homo sapiens] >ref|NP_001116212.1| estrogen receptor isoform 1 [Homo sapiens]
1241
estrogen receptor 1 [synthetic construct]
1241
oestrogen receptor [Homo sapiens] >gb|AAA52399.1| estrogen receptor [Homo sapiens] >dbj|BAH02304.1| estrogen receptor 1 [Homo sapiens]
1238
estrogen receptor isoform 2 [Homo sapiens]
1236
green fluorescent protein-estrogen receptor alpha fusion [Mammalian expression vector pCI-nGL1-HEGO]
1234
estrogen receptor isoform 3 [Homo sapiens]
1234
unnamed protein product [Homo sapiens]
1231
PREDICTED: estrogen receptor isoform 1 [Pan troglodytes] >ref|XP_003311597.1| PREDICTED: estrogen receptor isoform 2 [Pan troglodytes]
1229
PREDICTED: estrogen receptor isoform 1 [Pan paniscus] >ref|XP_003811544.1| PREDICTED: estrogen receptor isoform 2 [Pan paniscus]
1228
PREDICTED: estrogen receptor isoform 1 [Pongo abelii] >ref|XP_002817539.1| PREDICTED: estrogen receptor isoform 2 [Pongo abelii]
1227
PREDICTED: estrogen receptor isoform 1 [Macaca mulatta] >ref|XP_002803904.1| PREDICTED: estrogen receptor isoform 2 [Macaca mulatta]
1227
estrogen receptor [Papio anubis] >gb|ABY64720.1| estrogen receptor alpha [Papio anubis]
1225
estrogen receptor alpha [Trachypithecus obscurus]
1223
estrogen receptor alpha [Chlorocebus aethiops]
1222
PREDICTED: estrogen receptor isoform 1 [Nomascus leucogenys]
1221
estrogen receptor alpha [Colobus guereza]
1220
estrogen receptor alpha [Hylobates lar]
1219
PREDICTED: estrogen receptor isoform 2 [Callithrix jacchus]
1209
estrogen receptor alpha [Cebus apella]
1208
estrogen receptor alpha [Ateles paniscus]
1206
PREDICTED: estrogen receptor isoform 1 [Callithrix jacchus]
1202
PREDICTED: estrogen receptor [Odobenus rosmarus divergens]
1162
PREDICTED: estrogen receptor isoform X7 [Mustela putorius furo] >ref|XP_004784393.1
1149
estrogen receptor [Bos taurus] >sp|P49884.3|ESR1_BOVIN RecName: Full=Estrogen receptor
1146
PREDICTED: estrogen receptor isoform X1 [Mustela putorius furo] >ref|XP_004753629.1
1144
PREDICTED: LOW QUALITY PROTEIN: estrogen receptor [Bubalus bubalis]
1144
estrogen receptor 1 [Bos grunniens]
1142
PREDICTED: estrogen receptor isoform 1 [Orcinus orca] >ref|XP_004263756.1
1132
estrogen receptor [Felis catus] >sp|Q53AD2.1|ESR1_FELCA RecName: Full=Estrogen receptor
1127
PREDICTED: estrogen receptor [Lipotes vexillifer]
1126
PREDICTED: estrogen receptor [Otolemur garnettii]
1126
estrogen receptor [Sus scrofa] >sp|Q29040.1|ESR1_PIG RecName: Full=Estrogen receptor;
1122
estrogen receptor alpha [Sus scrofa]
1115
PREDICTED: estrogen receptor isoform X2 [Ictidomys tridecemlineatus]
1110
PREDICTED: estrogen receptor isoform X1 [Pteropus alecto]
1106
PREDICTED: estrogen receptor isoform X1 [Ictidomys tridecemlineatus]
1105
PREDICTED: estrogen receptor isoform X3 [Ictidomys tridecemlineatus]
1103
PREDICTED: estrogen receptor alpha-like [Oryctolagus cuniculus]
1102
PREDICTED: estrogen receptor isoform X2 [Pteropus alecto]
1099
PREDICTED: estrogen receptor isoform 1 [Trichechus manatus latirostris]
1096
estrogen receptor alpha [Rhinoceros unicornis]
1095
estrogen receptor [Equus caballus] >sp|Q9TV98.1|ESR1_HORSE RecName: Full=Estrogen receptor;
1094
estrogen receptor alpha variant U2 [Mus musculus] >gb|ACF17958.1| estrogen receptor alpha variant U1 [Mus musculus]
1093
estrogen receptor [Tupaia chinensis]
1092
estrogen receptor [Canis lupus familiaris] >ref|XP_005615579.1| PREDICTED: estrogen receptor isoform X2 [Canis lupus familiaris]
1092
estrogen receptor [Mus musculus] >ref|XP_006512495.1| PREDICTED: estrogen receptor isoform X1 [Mus musculus] >ref|XP_006512496.1
1092
Estrogen receptor [Pteropus alecto]
1090
PREDICTED: estrogen receptor isoform 2 [Trichechus manatus latirostris]
1089
PREDICTED: estrogen receptor isoform X5 [Tupaia chinensis]
1085
PREDICTED: estrogen receptor isoform X1 [Tupaia chinensis] >ref|XP_006140065.1
1079
PREDICTED: estrogen receptor [Ochotona princeps]
1076
PREDICTED: estrogen receptor isoform X2 [Condylura cristata]
1074
rCG41136, isoform CRA_b [Rattus norvegicus]
1071
PREDICTED: estrogen receptor isoform X1 [Rattus norvegicus)
1071
PREDICTED: estrogen receptor-like [Monodelphis domestica]
1070
PREDICTED: estrogen receptor isoform X2 [Rattus norvegicus]
1070
PREDICTED: estrogen receptor isoform X3 [Rattus norvegicus] >ref|XP_006227894.1
1070
PREDICTED: estrogen receptor isoform X1 [Condylura cristata]
1069
PREDICTED: estrogen receptor isoform X3 [Condylura cristata]
1067
PREDICTED: estrogen receptor [Sarcophilus harrisii]
1066
estrogen receeptor [Rattus norvegicus]
1066
80 kDa estrogen receptor [Homo sapiens]
1065
estrogen receptor [Rattus norvegicus] >sp|P06211.1|ESR1_RAT RecName: Full=Estrogen receptor; Short=ER
1064
PREDICTED: estrogen receptor [Chrysochloris asiatica]
1058
PREDICTED: estrogen receptor-like [Heterocephalus glaber]
1056
PREDICTED: estrogen receptor isoform X2 [Octodon degus] >ref|XP_004646043.1
1053
estrogen receptor 1 [Ceratotherium simum simum] >gb|AEY83589.1
1052
PREDICTED: estrogen receptor isoform X1 [Octodon degus]
1048
PREDICTED: estrogen receptor [Cavia porcellus]
1047
PREDICTED: estrogen receptor isoform X4 [Octodon degus]
1046
estrogen receptor [Mesocricetus auratus] >sp|Q9QZJ5.1|ESR1_MESAU RecName: Full=Estrogen receptor
1045
PREDICTED: estrogen receptor [Elephantulus edwardii]
1043
PREDICTED: estrogen receptor [Peromyscus maniculatus bairdii]
1042
unnamed protein product [Homo sapiens]
1041
PREDICTED: estrogen receptor isoform 1 [Dasypus novemcinctus] >ref|XP_004460436.1
1035
estrogen receptor 1 [Microtus ochrogaster] >gb|AAY53653.1| estrogen receptor alpha [Microtus ochrogaster]
1032
PREDICTED: estrogen receptor, partial [Myotis lucifugus]
1026
PREDICTED: estrogen receptor [Cricetulus griseus]
1026
PREDICTED: estrogen receptor [Sorex araneus]
1024
PREDICTED: estrogen receptor isoform X1 [Chinchilla lanigera] >ref|XP_005390631.1
1021
PREDICTED: estrogen receptor [Jaculus jaculus]
1015
PREDICTED: estrogen receptor [Balaenoptera acutorostrata scammoni]
1014
PREDICTED: estrogen receptor isoform X4 [Ictidomys tridecemlineatus]
1004
hypothetical protein PANDA_009027 [Ailuropoda melanoleuca]
1000
PREDICTED: estrogen receptor [Leptonychotes weddellii]
991
PREDICTED: estrogen receptor isoform X2 [Pelodiscus sinensis] >ref|XP_006115037.1
990
estrogen receptor alpha [Pseudemys nelsoni]
990
PREDICTED: estrogen receptor isoform X1 [Pelodiscus sinensis]
989
PREDICTED: estrogen receptor-like [Meleagris gallopavo]
984
estrogen receptor alpha [Lepidochelys olivacea]
983
estrogen receptor 1 [Chrysemys picta] >gb|ACI23369.1| estrogen receptor [Chrysemys picta]
983
PREDICTED: estrogen receptor isoform X2 [Chelonia mydas]
981
estrogen receptor alpha [Torgos tracheliotus]
981
estrogen receptor alpha [Gyps africanus]
981
PREDICTED: estrogen receptor [Anas platyrhynchos] >gb|EOA97541.1| Estrogen receptor, partial [Anas platyrhynchos]
981
PREDICTED: estrogen receptor isoform X1 [Chelonia mydas]
980
estrogen receptor alpha [Crocodylus niloticus]
979
estrogen receptor alpha [Gallus gallus]
979
estrogen receptor [Gallus gallus] >sp|P06212.1|ESR1_CHICK RecName: Full=Estrogen receptor; Short=ER
977
estrogen receptor alpha [Coturnix japonica]
976


I.TUGAS
Kode Gen       : NM_00693.1
Nama Gen       : Homosapien. Palgmerae (DNA Directed) Gamma (Polgi) Transcrip
CDS                : 2034002
Frame
Panjang
Start Kodon
Stop Kodon
+1
1 .... 3719
34
TAG
+2
1403 .... 1510
2
TGA
+2
1823 .... 2005
2
TGA
+2
2201 .... 2392
5
TGA
+2
3377 .... 3478
2
TGA
+3
3 ....728
-
TGA
-1
1 ...186
-
-
-1
1105 ... 1212
1
TAA
-1
2149 ....2412
2
TAG
-1
2455 ... 2733
1
TGA
-1
3526 .... 3719
-
TGA
-2
1 ....107
-
-
-2
447 .... 644
2
TAG
-2
828 .... 1049
6
TGA
-2
1086 ... 1280
3
TAA
-2
1353 .... 1574
2
TAA
-2
1617 .... 1808
2
TGA
-2
1944 .... 2060
2
TAG
-2
2508 .... 2630
3
TAG
-2
2649 .... 2822
2
TAG
-2
2862 .... 3236
3
TAG
-3
1 .... 631
2
-
-3
833 .... 1023
1
TGA
-3
1628 .... 1753
1
TGA
-3
2918 .... 3145
1
TGA


(HOMOLOGI PROTEIN)
Kode Gen       : NM_
Nama Gen       : NP_002684
CDS                : 1 ....1239

Description
Max Score
DNA polymerase subunit gamma-1 [Homo sapiens] >ref|NP_001119603.1| DNA polymerase subunit gamma-1 [Homo sapiens] >sp|P54098.1
2559
DNA polymerase subunit gamma-1 [Homo sapiens]
2557
DNA polymerase subunit gamma-1 [Homo sapiens)
2557
unnamed protein product [Homo sapiens]
2555
mitochondrial DNA polymerase gamma [Homo sapiens]
2554
polymerase (DNA directed), gamma [Homo sapiens]
2554
DNA polymerase subunit gamma-1 [Homo sapiens]
2554
PREDICTED: DNA polymerase subunit gamma-1 [Pan paniscus]
2534
PREDICTED: DNA polymerase subunit gamma-1 [Pan troglodytes]
2533
PREDICTED: DNA polymerase subunit gamma-1 [Gorilla gorilla gorilla]
2529
PREDICTED: DNA polymerase subunit gamma-1 [Nomascus leucogenys]
2472
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Papio anubis]
2457
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Macaca fascicularis]
2447
hypothetical protein EGK_17816 [Macaca mulatta]
2447
PREDICTED: DNA polymerase subunit gamma-1 [Macaca mulatta]
2446
hypothetical protein EGM_16268 [Macaca fascicularis]
2437
Chain A, Crystal Structure Of Human Mitochondrial Dna Polymerase Holoenzyme >pdb|3IKM|D Chain D, Crystal Structure Of Human Mitochondrial Dna
2417
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Pongo abelii]
2403
polymerase (DNA directed), gamma, isoform CRA_b [Homo sapiens]
2393
PREDICTED: DNA polymerase subunit gamma-1 [Callithrix jacchus]
2348
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Saimiri boliviensis boliviensis]
2342
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Macaca fascicularis]
2310
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Macaca fascicularis]
2281
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Otolemur garnettii]
2261
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Orcinus orca]
2254
PREDICTED: DNA polymerase subunit gamma-1 [Ceratotherium simum simum]
2254
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Lipotes vexillifer]
2248
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Equus caballus]
2243
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Ictidomys tridecemlineatus]
2241
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Balaenoptera acutorostrata scammoni]
2233
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Mustela putorius furo] >ref|XP_004798664.1
2207
PREDICTED: DNA polymerase subunit gamma-1 [Loxodonta africana]
2207
TPA: polymerase (DNA directed), gamma [Bos taurus]
2207
PREDICTED: DNA polymerase subunit gamma-1 [Jaculus jaculus]
2202
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Sus scrofa] >ref|XP_005653577.1|
2202
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Bubalus bubalis]
2200
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Physeter catodon]
2196
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Equus caballus]
2196
PREDICTED: DNA polymerase subunit gamma-1 isoform 1 [Dasypus novemcinctus]
2194
PREDICTED: DNA polymerase subunit gamma-1 [Trichechus manatus latirostris]
2193
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1-like [Ailuropoda melanoleuca]
2184
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Physeter catodon]
2184
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Tupaia chinensis]   
2184
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Bos taurus] >ref|XP_002696610.2
2174
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Heterocephalus glaber] >ref|XP_004852816.1
2174
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Canis lupus familiaris]
2173
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Felis catus]
2172
PREDICTED: DNA polymerase subunit gamma-1 [Chrysochloris asiatica]
2168
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Odobenus rosmarus divergens]
2166
PREDICTED: DNA polymerase subunit gamma-1 [Ochotona princeps]
2162
PREDICTED: DNA polymerase subunit gamma-1 [Heterocephalus glaber)
2160
DNA polymerase subunit gamma-1 [Heterocephalus glaber]
2155
hypothetical protein PANDA_008249 [Ailuropoda melanoleuca]
2154
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Octodon degus]
2152
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Octodon degus]
2152
PREDICTED: DNA polymerase subunit gamma-1-like [Cricetulus griseus] >gb|EGW10314.1
2152
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Cavia porcellus]
2151
unnamed protein product [Mus musculus]
2150
PREDICTED: DNA polymerase subunit gamma-1 [Mesocricetus auratus]
2150
DNA polymerase subunit gamma-1 [Mus musculus] >dbj|BAC98463.1| polymerase (DNA directed),
2149
polymerase (DNA directed), gamma, isoform CRA_b [Mus musculus]
2148
unnamed protein product [Mus musculus]
2144
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Microtus ochrogaster] >ref|XP_005357785.1
2143
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Rattus norvegicus] >ref|XP_006229473.1
2138
DNA polymerase subunit gamma-1 [Rattus norvegicus] >emb|CAB56207.1| DNA polymerase gamma
2137
PREDICTED: DNA polymerase subunit gamma-1 [Peromyscus maniculatus bairdii]
2130
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Leptonychotes weddellii)
2128
DNA polymerase subunit gamma-1 [Tupaia chinensis]
2127
PREDICTED: DNA polymerase subunit gamma-1 [Elephantulus edwardii]
2117
PREDICTED: DNA polymerase subunit gamma-1 [Chinchilla lanigera]
2114
PREDICTED: DNA polymerase subunit gamma-1 [Echinops telfairi]
2097
RecName: Full=DNA polymerase subunit gamma-1; AltName: Full=Mitochondrial DNA polymerase catalytic subunit
2095
PREDICTED: DNA polymerase subunit gamma-1 [Pteropus alecto]
2094
DNA polymerase subunit gamma-1 [Pteropus alecto]
2083
PREDICTED: DNA polymerase subunit gamma-1 [Myotis lucifugus]
2077
PREDICTED: DNA polymerase subunit gamma-1 isoform 2 [Dasypus novemcinctus]
2065
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Sus scrofa]
2061
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1, partial [Bos mutus]
2050
PREDICTED: DNA polymerase subunit gamma-1 [Condylura cristata]
2049
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Mus musculus]
2037
DNA polymerase gamma [Mus musculus]
2034
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Rattus norvegicus]
1978
PREDICTED: DNA polymerase subunit gamma-1 [Vicugna pacos]
1976
PREDICTED: DNA polymerase subunit gamma-1 [Sorex araneus]
1910
DNA polymerase subunit gamma-1 [Camelus ferus]
1910
PREDICTED: DNA polymerase subunit gamma-1 [Pantholops hodgsonii]
1844
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Ovis aries]
1838
PREDICTED: DNA polymerase subunit gamma-1-like [Monodelphis domestica]
1831
PREDICTED: DNA polymerase subunit gamma-1 [Sarcophilus harrisii]
1800
PREDICTED: LOW QUALITY PROTEIN: polymerase (DNA directed), gamma [Capra hircus]
1782
PREDICTED: DNA polymerase subunit gamma-1 [Chrysemys picta bellii]
1781
PREDICTED: DNA polymerase subunit gamma-1 [Camelus ferus]
1779
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Pelodiscus sinensis]
1772
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Pelodiscus sinensis]
1767
PREDICTED: LOW QUALITY PROTEIN: polymerase (DNA directed), gamma [Panthera tigris altaica]
1765
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Columba livia]
1765
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Columba livia]
1763
Polg protein [Mus musculus]
1743
DNA polymerase subunit gamma-1, partial [Columba livia]
1738
PREDICTED: DNA polymerase subunit gamma-1 [Pseudopodoces humilis]
1733


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