Sabtu, 02 Juli 2016

PRAKTIKUM BIOLOGI SEL MOLEKULER ANALISIS GEN DAN HOMOLOGI PROTEIN


LAPORAN RESMI
PRAKTIKUM  BIOLOGI SEL MOLEKULER
PERCOBAAN 2
ANALISIS GEN DAN HOMOLOGI PROTEIN

I.TUJUAN
1. Mapu melakukan analisis terhadap ekspresi gen dan dapat mendeteksi hasil ekspresi gen
2. Dapat mencari homologi gen penghasil protein tertentu dari manusia dengan gen beberapa organisme lain
II. ALAT DAN BAHAN
1.      Modem
2.      Laptop
3.      Pulsa
III. HASIL PENGAMATAN
I.LATIHAN
Kode Gen       : NM_000125
Nama Gen       : Homosapiens Estrogen Receptor-1
CDS                : 235...2022
Frame
Panjang
Start Kodon
Stop Kodon
+1
1....1787
27
ATG
+2
506...751
4
TGA
+2
1610....1787
2
TGA
+3
3....254
-
TGA
+3
1248....1364
1
TAA
-1
1572....1294
2
TGA
-2
735....977
3
TAG
-2
1583...1583
9
TGA
-3
299....445
1
TGA

Kode Gen       :  NM_000125
Nama Gen       : Homosapiens Estrogen Receptor-1
Kode Protein   : NP_000162.2
CDS                : 1....595
DESKRIPSI
MAX SCORE
estrogen receptor isoform 1 [Homo sapiens] >ref|NP_001116212.1| estrogen receptor isoform 1 [Homo sapiens]
1241
estrogen receptor 1 [synthetic construct]
1241
oestrogen receptor [Homo sapiens] >gb|AAA52399.1| estrogen receptor [Homo sapiens] >dbj|BAH02304.1| estrogen receptor 1 [Homo sapiens]
1238
estrogen receptor isoform 2 [Homo sapiens]
1236
green fluorescent protein-estrogen receptor alpha fusion [Mammalian expression vector pCI-nGL1-HEGO]
1234
estrogen receptor isoform 3 [Homo sapiens]
1234
unnamed protein product [Homo sapiens]
1231
PREDICTED: estrogen receptor isoform 1 [Pan troglodytes] >ref|XP_003311597.1| PREDICTED: estrogen receptor isoform 2 [Pan troglodytes]
1229
PREDICTED: estrogen receptor isoform 1 [Pan paniscus] >ref|XP_003811544.1| PREDICTED: estrogen receptor isoform 2 [Pan paniscus]
1228
PREDICTED: estrogen receptor isoform 1 [Pongo abelii] >ref|XP_002817539.1| PREDICTED: estrogen receptor isoform 2 [Pongo abelii]
1227
PREDICTED: estrogen receptor isoform 1 [Macaca mulatta] >ref|XP_002803904.1| PREDICTED: estrogen receptor isoform 2 [Macaca mulatta]
1227
estrogen receptor [Papio anubis] >gb|ABY64720.1| estrogen receptor alpha [Papio anubis]
1225
estrogen receptor alpha [Trachypithecus obscurus]
1223
estrogen receptor alpha [Chlorocebus aethiops]
1222
PREDICTED: estrogen receptor isoform 1 [Nomascus leucogenys]
1221
estrogen receptor alpha [Colobus guereza]
1220
estrogen receptor alpha [Hylobates lar]
1219
PREDICTED: estrogen receptor isoform 2 [Callithrix jacchus]
1209
estrogen receptor alpha [Cebus apella]
1208
estrogen receptor alpha [Ateles paniscus]
1206
PREDICTED: estrogen receptor isoform 1 [Callithrix jacchus]
1202
PREDICTED: estrogen receptor [Odobenus rosmarus divergens]
1162
PREDICTED: estrogen receptor isoform X7 [Mustela putorius furo] >ref|XP_004784393.1
1149
estrogen receptor [Bos taurus] >sp|P49884.3|ESR1_BOVIN RecName: Full=Estrogen receptor
1146
PREDICTED: estrogen receptor isoform X1 [Mustela putorius furo] >ref|XP_004753629.1
1144
PREDICTED: LOW QUALITY PROTEIN: estrogen receptor [Bubalus bubalis]
1144
estrogen receptor 1 [Bos grunniens]
1142
PREDICTED: estrogen receptor isoform 1 [Orcinus orca] >ref|XP_004263756.1
1132
estrogen receptor [Felis catus] >sp|Q53AD2.1|ESR1_FELCA RecName: Full=Estrogen receptor
1127
PREDICTED: estrogen receptor [Lipotes vexillifer]
1126
PREDICTED: estrogen receptor [Otolemur garnettii]
1126
estrogen receptor [Sus scrofa] >sp|Q29040.1|ESR1_PIG RecName: Full=Estrogen receptor;
1122
estrogen receptor alpha [Sus scrofa]
1115
PREDICTED: estrogen receptor isoform X2 [Ictidomys tridecemlineatus]
1110
PREDICTED: estrogen receptor isoform X1 [Pteropus alecto]
1106
PREDICTED: estrogen receptor isoform X1 [Ictidomys tridecemlineatus]
1105
PREDICTED: estrogen receptor isoform X3 [Ictidomys tridecemlineatus]
1103
PREDICTED: estrogen receptor alpha-like [Oryctolagus cuniculus]
1102
PREDICTED: estrogen receptor isoform X2 [Pteropus alecto]
1099
PREDICTED: estrogen receptor isoform 1 [Trichechus manatus latirostris]
1096
estrogen receptor alpha [Rhinoceros unicornis]
1095
estrogen receptor [Equus caballus] >sp|Q9TV98.1|ESR1_HORSE RecName: Full=Estrogen receptor;
1094
estrogen receptor alpha variant U2 [Mus musculus] >gb|ACF17958.1| estrogen receptor alpha variant U1 [Mus musculus]
1093
estrogen receptor [Tupaia chinensis]
1092
estrogen receptor [Canis lupus familiaris] >ref|XP_005615579.1| PREDICTED: estrogen receptor isoform X2 [Canis lupus familiaris]
1092
estrogen receptor [Mus musculus] >ref|XP_006512495.1| PREDICTED: estrogen receptor isoform X1 [Mus musculus] >ref|XP_006512496.1
1092
Estrogen receptor [Pteropus alecto]
1090
PREDICTED: estrogen receptor isoform 2 [Trichechus manatus latirostris]
1089
PREDICTED: estrogen receptor isoform X5 [Tupaia chinensis]
1085
PREDICTED: estrogen receptor isoform X1 [Tupaia chinensis] >ref|XP_006140065.1
1079
PREDICTED: estrogen receptor [Ochotona princeps]
1076
PREDICTED: estrogen receptor isoform X2 [Condylura cristata]
1074
rCG41136, isoform CRA_b [Rattus norvegicus]
1071
PREDICTED: estrogen receptor isoform X1 [Rattus norvegicus)
1071
PREDICTED: estrogen receptor-like [Monodelphis domestica]
1070
PREDICTED: estrogen receptor isoform X2 [Rattus norvegicus]
1070
PREDICTED: estrogen receptor isoform X3 [Rattus norvegicus] >ref|XP_006227894.1
1070
PREDICTED: estrogen receptor isoform X1 [Condylura cristata]
1069
PREDICTED: estrogen receptor isoform X3 [Condylura cristata]
1067
PREDICTED: estrogen receptor [Sarcophilus harrisii]
1066
estrogen receeptor [Rattus norvegicus]
1066
80 kDa estrogen receptor [Homo sapiens]
1065
estrogen receptor [Rattus norvegicus] >sp|P06211.1|ESR1_RAT RecName: Full=Estrogen receptor; Short=ER
1064
PREDICTED: estrogen receptor [Chrysochloris asiatica]
1058
PREDICTED: estrogen receptor-like [Heterocephalus glaber]
1056
PREDICTED: estrogen receptor isoform X2 [Octodon degus] >ref|XP_004646043.1
1053
estrogen receptor 1 [Ceratotherium simum simum] >gb|AEY83589.1
1052
PREDICTED: estrogen receptor isoform X1 [Octodon degus]
1048
PREDICTED: estrogen receptor [Cavia porcellus]
1047
PREDICTED: estrogen receptor isoform X4 [Octodon degus]
1046
estrogen receptor [Mesocricetus auratus] >sp|Q9QZJ5.1|ESR1_MESAU RecName: Full=Estrogen receptor
1045
PREDICTED: estrogen receptor [Elephantulus edwardii]
1043
PREDICTED: estrogen receptor [Peromyscus maniculatus bairdii]
1042
unnamed protein product [Homo sapiens]
1041
PREDICTED: estrogen receptor isoform 1 [Dasypus novemcinctus] >ref|XP_004460436.1
1035
estrogen receptor 1 [Microtus ochrogaster] >gb|AAY53653.1| estrogen receptor alpha [Microtus ochrogaster]
1032
PREDICTED: estrogen receptor, partial [Myotis lucifugus]
1026
PREDICTED: estrogen receptor [Cricetulus griseus]
1026
PREDICTED: estrogen receptor [Sorex araneus]
1024
PREDICTED: estrogen receptor isoform X1 [Chinchilla lanigera] >ref|XP_005390631.1
1021
PREDICTED: estrogen receptor [Jaculus jaculus]
1015
PREDICTED: estrogen receptor [Balaenoptera acutorostrata scammoni]
1014
PREDICTED: estrogen receptor isoform X4 [Ictidomys tridecemlineatus]
1004
hypothetical protein PANDA_009027 [Ailuropoda melanoleuca]
1000
PREDICTED: estrogen receptor [Leptonychotes weddellii]
991
PREDICTED: estrogen receptor isoform X2 [Pelodiscus sinensis] >ref|XP_006115037.1
990
estrogen receptor alpha [Pseudemys nelsoni]
990
PREDICTED: estrogen receptor isoform X1 [Pelodiscus sinensis]
989
PREDICTED: estrogen receptor-like [Meleagris gallopavo]
984
estrogen receptor alpha [Lepidochelys olivacea]
983
estrogen receptor 1 [Chrysemys picta] >gb|ACI23369.1| estrogen receptor [Chrysemys picta]
983
PREDICTED: estrogen receptor isoform X2 [Chelonia mydas]
981
estrogen receptor alpha [Torgos tracheliotus]
981
estrogen receptor alpha [Gyps africanus]
981
PREDICTED: estrogen receptor [Anas platyrhynchos] >gb|EOA97541.1| Estrogen receptor, partial [Anas platyrhynchos]
981
PREDICTED: estrogen receptor isoform X1 [Chelonia mydas]
980
estrogen receptor alpha [Crocodylus niloticus]
979
estrogen receptor alpha [Gallus gallus]
979
estrogen receptor [Gallus gallus] >sp|P06212.1|ESR1_CHICK RecName: Full=Estrogen receptor; Short=ER
977
estrogen receptor alpha [Coturnix japonica]
976

I.TUGAS
Kode Gen       : NM_00693.1
Nama Gen       : Homosapien. Palgmerae (DNA Directed) Gamma (Polgi) Transcrip
CDS                : 2034002
Frame
Panjang
Start Kodon
Stop Kodon
+1
1 .... 3719
34
TAG
+2
1403 .... 1510
2
TGA
+2
1823 .... 2005
2
TGA
+2
2201 .... 2392
5
TGA
+2
3377 .... 3478
2
TGA
+3
3 ....728
-
TGA
-1
1 ...186
-
-
-1
1105 ... 1212
1
TAA
-1
2149 ....2412
2
TAG
-1
2455 ... 2733
1
TGA
-1
3526 .... 3719
-
TGA
-2
1 ....107
-
-
-2
447 .... 644
2
TAG
-2
828 .... 1049
6
TGA
-2
1086 ... 1280
3
TAA
-2
1353 .... 1574
2
TAA
-2
1617 .... 1808
2
TGA
-2
1944 .... 2060
2
TAG
-2
2508 .... 2630
3
TAG
-2
2649 .... 2822
2
TAG
-2
2862 .... 3236
3
TAG
-3
1 .... 631
2
-
-3
833 .... 1023
1
TGA
-3
1628 .... 1753
1
TGA
-3
2918 .... 3145
1
TGA








(HOMOLOGI PROTEIN)
Kode Gen       : NM_
Nama Gen       : NP_002684
CDS                : 1 ....1239

Description
Max Score
DNA polymerase subunit gamma-1 [Homo sapiens] >ref|NP_001119603.1| DNA polymerase subunit gamma-1 [Homo sapiens] >sp|P54098.1
2559
DNA polymerase subunit gamma-1 [Homo sapiens]
2557
DNA polymerase subunit gamma-1 [Homo sapiens)
2557
unnamed protein product [Homo sapiens]
2555
mitochondrial DNA polymerase gamma [Homo sapiens]
2554
polymerase (DNA directed), gamma [Homo sapiens]
2554
DNA polymerase subunit gamma-1 [Homo sapiens]
2554
PREDICTED: DNA polymerase subunit gamma-1 [Pan paniscus]
2534
PREDICTED: DNA polymerase subunit gamma-1 [Pan troglodytes]
2533
PREDICTED: DNA polymerase subunit gamma-1 [Gorilla gorilla gorilla]
2529
PREDICTED: DNA polymerase subunit gamma-1 [Nomascus leucogenys]
2472
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Papio anubis]
2457
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Macaca fascicularis]
2447
hypothetical protein EGK_17816 [Macaca mulatta]
2447
PREDICTED: DNA polymerase subunit gamma-1 [Macaca mulatta]
2446
hypothetical protein EGM_16268 [Macaca fascicularis]
2437
Chain A, Crystal Structure Of Human Mitochondrial Dna Polymerase Holoenzyme >pdb|3IKM|D Chain D, Crystal Structure Of Human Mitochondrial Dna
2417
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Pongo abelii]
2403
polymerase (DNA directed), gamma, isoform CRA_b [Homo sapiens]
2393
PREDICTED: DNA polymerase subunit gamma-1 [Callithrix jacchus]
2348
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Saimiri boliviensis boliviensis]
2342
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Macaca fascicularis]
2310
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Macaca fascicularis]
2281
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Otolemur garnettii]
2261
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Orcinus orca]
2254
PREDICTED: DNA polymerase subunit gamma-1 [Ceratotherium simum simum]
2254
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Lipotes vexillifer]
2248
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Equus caballus]
2243
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Ictidomys tridecemlineatus]
2241
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Balaenoptera acutorostrata scammoni]
2233
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Mustela putorius furo] >ref|XP_004798664.1
2207
PREDICTED: DNA polymerase subunit gamma-1 [Loxodonta africana]
2207
TPA: polymerase (DNA directed), gamma [Bos taurus]
2207
PREDICTED: DNA polymerase subunit gamma-1 [Jaculus jaculus]
2202
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Sus scrofa] >ref|XP_005653577.1|
2202
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Bubalus bubalis]
2200
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Physeter catodon]
2196
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Equus caballus]
2196
PREDICTED: DNA polymerase subunit gamma-1 isoform 1 [Dasypus novemcinctus]
2194
PREDICTED: DNA polymerase subunit gamma-1 [Trichechus manatus latirostris]
2193
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1-like [Ailuropoda melanoleuca]
2184
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Physeter catodon]
2184
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Tupaia chinensis]   
2184
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Bos taurus] >ref|XP_002696610.2
2174
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Heterocephalus glaber] >ref|XP_004852816.1
2174
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Canis lupus familiaris]
2173
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Felis catus]
2172
PREDICTED: DNA polymerase subunit gamma-1 [Chrysochloris asiatica]
2168
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Odobenus rosmarus divergens]
2166
PREDICTED: DNA polymerase subunit gamma-1 [Ochotona princeps]
2162
PREDICTED: DNA polymerase subunit gamma-1 [Heterocephalus glaber)
2160
DNA polymerase subunit gamma-1 [Heterocephalus glaber]
2155
hypothetical protein PANDA_008249 [Ailuropoda melanoleuca]
2154
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Octodon degus]
2152
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Octodon degus]
2152
PREDICTED: DNA polymerase subunit gamma-1-like [Cricetulus griseus] >gb|EGW10314.1
2152
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Cavia porcellus]
2151
unnamed protein product [Mus musculus]
2150
PREDICTED: DNA polymerase subunit gamma-1 [Mesocricetus auratus]
2150
DNA polymerase subunit gamma-1 [Mus musculus] >dbj|BAC98463.1| polymerase (DNA directed),
2149
polymerase (DNA directed), gamma, isoform CRA_b [Mus musculus]
2148
unnamed protein product [Mus musculus]
2144
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Microtus ochrogaster] >ref|XP_005357785.1
2143
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Rattus norvegicus] >ref|XP_006229473.1
2138
DNA polymerase subunit gamma-1 [Rattus norvegicus] >emb|CAB56207.1| DNA polymerase gamma
2137
PREDICTED: DNA polymerase subunit gamma-1 [Peromyscus maniculatus bairdii]
2130
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Leptonychotes weddellii)
2128
DNA polymerase subunit gamma-1 [Tupaia chinensis]
2127
PREDICTED: DNA polymerase subunit gamma-1 [Elephantulus edwardii]
2117
PREDICTED: DNA polymerase subunit gamma-1 [Chinchilla lanigera]
2114
PREDICTED: DNA polymerase subunit gamma-1 [Echinops telfairi]
2097
RecName: Full=DNA polymerase subunit gamma-1; AltName: Full=Mitochondrial DNA polymerase catalytic subunit
2095
PREDICTED: DNA polymerase subunit gamma-1 [Pteropus alecto]
2094
DNA polymerase subunit gamma-1 [Pteropus alecto]
2083
PREDICTED: DNA polymerase subunit gamma-1 [Myotis lucifugus]
2077
PREDICTED: DNA polymerase subunit gamma-1 isoform 2 [Dasypus novemcinctus]
2065
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Sus scrofa]
2061
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1, partial [Bos mutus]
2050
PREDICTED: DNA polymerase subunit gamma-1 [Condylura cristata]
2049
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Mus musculus]
2037
DNA polymerase gamma [Mus musculus]
2034
PREDICTED: DNA polymerase subunit gamma-1 isoform X3 [Rattus norvegicus]
1978
PREDICTED: DNA polymerase subunit gamma-1 [Vicugna pacos]
1976
PREDICTED: DNA polymerase subunit gamma-1 [Sorex araneus]
1910
DNA polymerase subunit gamma-1 [Camelus ferus]
1910
PREDICTED: DNA polymerase subunit gamma-1 [Pantholops hodgsonii]
1844
PREDICTED: LOW QUALITY PROTEIN: DNA polymerase subunit gamma-1 [Ovis aries]
1838
PREDICTED: DNA polymerase subunit gamma-1-like [Monodelphis domestica]
1831
PREDICTED: DNA polymerase subunit gamma-1 [Sarcophilus harrisii]
1800
PREDICTED: LOW QUALITY PROTEIN: polymerase (DNA directed), gamma [Capra hircus]
1782
PREDICTED: DNA polymerase subunit gamma-1 [Chrysemys picta bellii]
1781
PREDICTED: DNA polymerase subunit gamma-1 [Camelus ferus]
1779
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Pelodiscus sinensis]
1772
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Pelodiscus sinensis]
1767
PREDICTED: LOW QUALITY PROTEIN: polymerase (DNA directed), gamma [Panthera tigris altaica]
1765
PREDICTED: DNA polymerase subunit gamma-1 isoform X2 [Columba livia]
1765
PREDICTED: DNA polymerase subunit gamma-1 isoform X1 [Columba livia]
1763
Polg protein [Mus musculus]
1743
DNA polymerase subunit gamma-1, partial [Columba livia]
1738
PREDICTED: DNA polymerase subunit gamma-1 [Pseudopodoces humilis]
1733



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